The effect of Sarosate (N-phosphonomethyl glycine) a non-selective herbicide on the mycelial extension growth and sporulation of three soil fungi, (Aspergillius niger, Trichoderma sp. and Penicillium sp.) were investigated on Patotoe Dextrose Agar (PDA) containing 0, 0.01, 0.1, 0.5 and 1.0% v/v of the herbicides for a period of 96hours. The mycelial extension growth increased with time but decreased with concentration of the herbicide for the three fungi tested with growth occurring in all concentrations. Sporulation equally occurred in all tested fungi at 48 hours.